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Anti-Mouse CD25 eFluor® 450 (Pacific Blue® replacement)

Also known as: Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra

RUO: For Research Use Only

mouse CD25 antibody, eFluor® 450 conjugate

Staining of C57Bl/6 splenocytes with Anti-Mouse CD4 FITC (cat. 11-0041) and 0.25 μg of Rat IgG1 κ Isotype Control eFluor® 450 (cat. 48-4301) (left) or 0.25 μg of Anti-Mouse CD25 eFluor® 450 (right). Total viable cells were used for analysis.

Contents: Anti-Mouse CD25 eFluor® 450 (Pacific Blue® replacement)
Catalog Number: 48-0251
Concentration: 0.2 mg/mL
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material.
Clone: PC61.5
Host/Isotype: Rat IgG1, λ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
48-0251-80	25 ug	To view relevant pricing, please select your country and resume browsing.
48-0251-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
48-4301	Rat IgG1 K Isotype Control eFluor® 450 (Pacific Blue® replacement)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-0251	Anti-Mouse CD25 PE	488	575	FC	RUO
13-0251	Anti-Mouse CD25 Biotin	N/A	N/A	FC	RUO
14-0251	Anti-Mouse CD25 Purified	N/A	N/A	FC IHC IP	RUO
15-0251	Anti-Mouse CD25 PE-Cy5	488	670	FC	RUO
16-0251	Anti-Mouse CD25 Functional Grade* Purified	N/A	N/A	FA FC	RUO
17-0251	Anti-Mouse CD25 APC	633	660	FC	RUO
25-0251	Anti-Mouse CD25 PE-Cy7	488	760	FC	RUO
36-0251	Anti-Mouse CD25 Functional Grade* Biotin	N/A	N/A	FC	RUO
45-0251	Anti-Mouse CD25 PerCP-Cy5.5	488	690	FC	RUO
47-0251	Anti-Mouse CD25 APC-eFluor® 780	633	780	FC	RUO
48-0251	Anti-Mouse CD25 eFluor® 450 (Pacific Blue® replacement)	405	450	FC	RUO
53-0251	Anti-Mouse CD25 Alexa Fluor® 488	488	519	FC	RUO
56-0251	Anti-Mouse CD25 Alexa Fluor® 700	633	723	FC	RUO
eFluor® products are not offered for sale or use in certain regions. Please contact an eBioscience representative for product availability.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µL to 5 µL. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This PC61.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 456 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

References

Tuve S, Chen BM, Liu Y, Cheng TL, Touré P, Sow PS, Feng Q, Kiviat N, Strauss R, Ni S, Li ZY, Roffler SR, Lieber A. Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses. Cancer Res. 2007 Jun 15;67(12):5929-39. (PC61.5, in vivo depletion, PubMed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (PC61.5, in vivo depletion, PubMed)

Hayashi T, Hasegawa K, Adachi C. Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Pathol. 2005 Oct;86(5):289-96. (PC61.5, in vivo depletion, PubMed)

Lowenthal JW, Corthésy P, Tougne C, Lees R, MacDonald HR, Nabholz M. High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J Immunol. 1985 Dec;135(6):3988-94.

Lowenthal JW, Zubler RH, Nabholz M, MacDonald HR. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature. 1985 Jun 20-26;315(6021):669-72.

Lowenthal JW, Tougne C, MacDonald HR, Smith KA, Nabholz M. Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. J Immunol. 1985 Feb;134(2):931-9.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.