eBioscience produces premium-quality yet value-priced immunological reagents that give you optimal signal-to-noise in your experiments. Our product expertise includes commonly
used immunophenotyping markers (mouse and human CDs) and cytokine reagents, as well as novel mAbs and conjugates to antigens such as TLR, costimulatory molecules
(e.g. PD-L1, PD-L2, ICOS, PD-1), and apoptosis molecules (DR, Fas, TWEAK, TRAIL, cytochrome C).
Below are protocols for our products which we have optimized to support their use. If we can be of further assistance, please contact us. Thank you for visiting eBioscience.
 Absolute S specific instructions, flow
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Flow cytometry assay protocol for measuring cell proliferation without any heat or acid-denaturation steps. Surface and internal epitopes are preserved.
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| Antigen surface staining, flow cytometry |
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ApoBrdU specific instructions, flow
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A two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.
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| ApoDirect specific instructions, flow |
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A two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. |
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| Apoptosis induction, using EOS9.1 |
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CD95 (Fas)-induced apoptosis of human Jurkat cell line by monoclonal antibody EOS9.1 is used as a general positive control for apoptosis detection systems. |
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| Baculovirus Protein Expression & Analysis |
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| Bioassays, cytokine |
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Measurement of biological activity of cytokines and their concentrations by cellular proliferation of primary cell cultures or isolated cell lines that are dependent
and/or responsive to a specific growth factor. |
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Common Fluorescent Dyes in Flow Cytometry |
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Cross-Reactivity of Anti-Human/Mouse Antibodies |
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Cytokine ELISA, antibody pairs |
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Used for the detection and quantification of cytokines secreted into tissue culture supernatant and/or biological fluids. Using two antibodies specific
for non-overlapping epitopes allows the optimal capture and detection of the desired cytokine. |
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Cytokine intracellular staining,
flow cytometry
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A modification of the basic immunofluorescence staining and flow cytometric analysis used for the simultaneous analysis of surface molecules and
intracellular cytokines at single-cell level. |
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Cytokine neutralizing protocols
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Protocols describe in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies. |
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Cytokine ELISA Ready-SET-Go! Kits (w/ pre-coated plates) Protocol |
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Cytokine ELISA Ready-SET-Go! Protocol |
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eFluor® Nanocrystals Surface Staining, Flow Cytometry
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eFluor® Nanocrystals Immunofluorescence Microscopy
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eFluor® Nanocrystals (Reactive) Conjugation
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eFluor® Nanocrystals in vivo Imaging
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eFluor® Nanocrystals Cell Labeling
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ELISPOT (Enzyme-linked ImmunoSPOT) |
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Used to obtain colorimetric footprint of B cells that can be quantitated by visual scoring or specialized plate-readers. |
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Foxp3 Protocol |
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Immunohistochemistry (IHC) Staining,
Frozen Tissues
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Immunoprecipitation Protocol |
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This technique provides a rapid and simple means to separate a specific protein from whole cell lysates or culture supernatants.
Additionally, immunoprecipitation can be used to confirm the identity or study biochemical characteristics, post-translational modifications, and expression levels of a protein of interest. |
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In Vivo Cytokine Capture Assay (IVCCA)
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In vivo use of antibodies |
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Antibodies are used for in vivo studies where the injected antibody is intended to either deplete a specific cell type or induce activation of cells.
eBioscience offers monoclonal antibodies to mouse cell surface antigens and cytokines for in vitro or in vivo functional studies. |
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T Cell Activation using anti-CD3 mAb
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Protocol A: Stimulation of mouse peripheral T cells with plate-bound 145-2C11 monoclonal antibody; MTT assay for detection of cellular proliferation.
Protocol B: Stimulation of human peripheral blood mononuclear cells with anti-human CD3 monoclonal antibody; MTT assay for detection of cellular proliferation. |
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| RBC Lysis |
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Thawing &
Freezing Protocols for insect cells (BV)
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Toll-Like Receptor (TLR) Detection, flow cytometry |
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Toll-Like Receptor 2 (TLR2) Neutralization Bioassay |
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Toll-Like Receptor 4 (TLR4) Neutralization Bioassay |
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TLR4 antibodies are used to inhibit the LPS-induced cytokine production by human PBMCs. |
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Immunoblotting Protocol (Western Blot)
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