Product Information
Contents: Alexa Fluor® 647 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)
Catalog Number: 51-9917
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
Clone: HTA125
Isotype: Mouse IgG2a, κ
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Staining of human TLR4-transfected MD5 cells (left) and normal human peripheral blood cells (right) with staining buffer (autofluorescence) (open histogram) or Alexa Fluor® 647 anti-human TLR4 (HTA125) (colored histogram). Total viable cells (left) or cells in the monocyte gate (right) were used for analysis.
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Available Formats of This Product |
Cat. No. |
Format |
Excite (nm) |
Emit (nm) |
Reported Applications |
14-9917 |
Affinity Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
N/A |
N/A |
FA
FC
IHC(Paraffin)
IP
|
16-9917 |
Functional Grade* Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
N/A |
N/A |
FA
FC
|
13-9917 |
Biotin anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
N/A |
N/A |
FC
|
12-9917 |
PE anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
488 |
575 |
FC
|
17-9917 |
APC anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
633 |
660 |
FC
|
25-9917 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
488 |
760 |
FC
|
51-9917 |
Alexa Fluor® 647 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
633 |
668 |
FC
|
53-9917 |
Alexa Fluor® 488 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
488 |
519 |
FC
|
56-9917 |
Alexa Fluor® 700 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
57-9917 |
Pacific Blue® anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors. For more information about detection of TLRs by flow cytometric analysis, please visit our website at
http://www.ebioscience.com/ebioscience/appls/TLR_FC.htm.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This HTA125 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This HTA125 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1.0 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
It is highly recommended that for optimal staining of TLR4, whole blood be stained using the
LWB protocol rather than Ficoll-gradient prepared PBMCs. The use of a density gradient appears to reduce the staining intensity significantly.
References
Tabeta, K., K. Yamazaki, et al. 2000. Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts. Infect Immun. 68(6): 3731-5.
Akashi, S., H. Ogata, et al. 2000. Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2. Biochem Biophys Res Commun. 268(1): 172-7.
Shimazu, R., S. Akashi, et al. 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med. 189(11): 1777-82.
de Graaf R, Kloppenburg G, et al. 2006. Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4. Microbes Infect. 8(7):1859-65. (HTA125, IHC paraffin,
PubMed)
Mirlashari MR and Lyberg T. 2003. Expression and involvement of Toll-like receptors (TLR)2, TLR4, and CD14 in monocyte TNF-alpha production induced by lipopolysaccharides from Neisseria meningitidis. Med Sci Monit. 9(8):BR316-24. (HTA125, FA,
PubMed)
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Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.