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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors. For more information about detection of TLRs by flow cytometric analysis, please visit our website at http://www.ebioscience.com/ebioscience/appls/TLR_FC.htm. Applications ReportedFor research use only, not for diagnostic or therapeutic use. This HTA125 antibody has been reported for use in flow cytometric analysis. This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light. Applications TestedThis HTA125 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1.0 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume). References
Tabeta, K., K. Yamazaki, et al. (2000). "Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts." Infect Immun 68(6): 3731-5. Akashi, S., H. Ogata, et al. (2000). "Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2." Biochem Biophys Res Commun 268(1): 172-7. Shimazu, R., S. Akashi, et al. (1999). "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." J Exp Med 189(11): 1777-82. Related ProductsCat. 25-4724 Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG2a, K Isotype Control Cat. 14-8185 Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor) Cat. 34-8185 Carrier-Free Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor) Cat. 11-8317 Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 (clone 225.C) Cat. 12-8317 Phycoerythrin (PE) mouse anti-human IFN-alpha2 (clone 225.C) Cat. 17-8317 Allophycocyanin (APC) mouse anti-human IFN-alpha2 (clone 225.C) Cat. 14-9920 Affinity Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) Polyclonal (clone Polyclonal) FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. Sale, lease, license or other transfer of the material or any material derived or produced from it. 2. Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it 3. Use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED. |
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