anti-human TLR4 antibody: Human Toll-like receptor 4 (TLR-4) antibody, no azide, azide free

Contents: Functional Grade Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)
Catalog Number: 16-9917
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: HTA125
Isotype: Mouse IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
16-9917-82	100 ug	$225

*International customers: Please contact your distributor for region specific pricing.

FG Purified HTA125 vs. FG Purified Mouse IgG2a Isotype Control
(Gated on normal peripheral monocytes)
Neutralization data can be found below

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-9917	PE anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	575	FC
13-9917	Biotin anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FC
14-9917	Affinity Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FA FC IHC(Paraffin) IP
16-9917	Functional Grade* Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FA FC
17-9917	APC anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	633	660	FC
25-9917	Phycoerythrin-Cy7 (PE-Cy7) anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	760	FC
51-9917	Alexa Fluor® 647 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	633	668	FC
53-9917	Alexa Fluor® 488 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	519	FC
56-9917	Alexa Fluor® 700 anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) (APC-Cy5.5 Equivalent)	633	723	FC
57-9917	Pacific Blue® anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors. For more information about detection of TLRs by flow cytometric analysis, please visit our website at http://www.ebioscience.com/ebioscience/appls/TLR_FC.htm.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HTA125 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of LPS-induced cytokine production. For detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti-mouse IgG and streptavidin-PE. For a detailed neutralization protocol for TLR4, please visit our website at: http://www.ebioscience.com/ebioscience/appls/NUtlr4.htm.

Applications Tested

The HTA125 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Tabeta, K., K. Yamazaki, et al. 2000. Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts. Infect Immun. 68(6): 3731-5.

Akashi, S., H. Ogata, et al. 2000. Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2. Biochem Biophys Res Commun. 268(1): 172-7.

Shimazu, R., S. Akashi, et al. 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med. 189(11): 1777-82.

de Graaf R, Kloppenburg G, et al. 2006. Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4. Microbes Infect. 8(7):1859-65. (HTA125, IHC paraffin, PubMed)

Mirlashari MR and Lyberg T. 2003. Expression and involvement of Toll-like receptors (TLR)2, TLR4, and CD14 in monocyte TNF-alpha production induced by lipopolysaccharides from Neisseria meningitidis. Med Sci Monit. 9(8):BR316-24. (HTA125, FA, PubMed)

Related Products

Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 16-4724    Functional Grade Purified Mouse IgG2a, K Isotype Control
Cat. 14-8185    Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor)
Cat. 34-8185    Carrier-Free Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor)
Cat. 11-8317    Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 12-8317    Phycoerythrin (PE) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 17-8317    Allophycocyanin (APC) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 14-9920    Affinity Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) Polyclonal (clone Polyclonal)