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Red Blood Cell (RBC) Lysis |
Introduction
Prior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to remove red blood cells. The eBioscience 1X RBC Lysis Buffer (Cat. No. 00-4333) is formulated for optimal lysis of erythrocytes in single cell suspensions of mouse tissues such as spleen and human peripheral blood. The buffer contains ammonium chloride, which lyses red cells with minimal effect on lymphocytes. This step is not necessary when working with mouse thymus and lymph nodes. RBC Lysis of Mouse SpleenMaterials
Instruments
Experiment Duration
Method
Note: In general, a small number of residual red cells does not interfere with proliferation assays or can be gated out from flow cytometric analysis. However, if required, a second round of lysis can be performed. |
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