Kinetic properties of rabbit testicular lactate dehydrogenase isozyme
LJ Battellino, FR Jaime, A Blanco - Journal of Biological Chemistry, 1968 - Elsevier
LJ Battellino, FR Jaime, A Blanco
Journal of Biological Chemistry, 1968•ElsevierThe additional lactate dehydrogenase isozyme from rabbit testis and sperm, lactate
dehydrogenase isozyme-1 (B 4) from heart, and lactate dehydrogenase isozyme-5 (A 4)
from liver have been separated and partially purified by preparative starch gel
electrophoresis. Studies performed on these fractions comprised K m and optimum substrate
concentration for pyruvate, α-ketobutyrate, and lactate, inhibition by substrate and product,
effect of oxamate, oxalate, urea, some citric acid cycle metabolites, pH, and heating. Results …
dehydrogenase isozyme-1 (B 4) from heart, and lactate dehydrogenase isozyme-5 (A 4)
from liver have been separated and partially purified by preparative starch gel
electrophoresis. Studies performed on these fractions comprised K m and optimum substrate
concentration for pyruvate, α-ketobutyrate, and lactate, inhibition by substrate and product,
effect of oxamate, oxalate, urea, some citric acid cycle metabolites, pH, and heating. Results …
The additional lactate dehydrogenase isozyme from rabbit testis and sperm, lactate dehydrogenase isozyme-1 (B4) from heart, and lactate dehydrogenase isozyme-5 (A4) from liver have been separated and partially purified by preparative starch gel electrophoresis.
Studies performed on these fractions comprised Km and optimum substrate concentration for pyruvate, α-ketobutyrate, and lactate, inhibition by substrate and product, effect of oxamate, oxalate, urea, some citric acid cycle metabolites, pH, and heating.
Results indicate that rabbit testicular lactate dehydrogenase isozyme is a distinct molecular form with peculiar catalytic properties. It showed different behavior against high substrate concentrations whether the direct or the reverse reaction was studied: striking inhibition by pyruvate, no effect with lactate. There is a remarkable correlation between these properties and metabolic requirements of mature spermatozoa.
Elsevier
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